05-1531

Anti-TATA-box-binding protein Antibody, clone SL30-3-563

clone SL30-3-563, from mouse

• Synonyme(s) : TATA box binding protein, Transcription initiation factor TFIID TBP subunit, TATA-binding factor, TATA sequence-binding protein, TATA-box binding protein N-terminal domain, TATA-box factor
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: SL30-3-563, monoclonal
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• Technique(s): ChIP: suitable; western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_003185
• Numéro d'accès UniProt: P20226
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... TBP(6908)

Description

Description générale

The TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.

Spécificité

This antibody recognizes TATA-box-binding protein at the N-terminus.

Immunogène

Epitope: N-terminus

GST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.

Application

Chromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initiation factor TFIID TBP subunit.

Qualité

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.

Description de la cible

~38 kDa observed.

Forme physique

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HeLa cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable