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906468

Sigma-Aldrich

SLAM-VproS-13CHD2 Methyl Labeling Kit

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.12

Technique(s)

bio NMR: suitable

Niveau de qualité

200

Conditions d'expédition

dry ice

Température de stockage

−70°C

Catégories apparentées

Description générale

SLAM-VproS-13CHD2 kit has 13CHD2 isotopomer precursor and contains protocol instructions for creation of isotopically-labeled proteins.

Application

For solid-state NMR applications and dynamic studies.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
SLAM-VproS-13CHD2 kit is used for stereospecific labeling of valine with 13CHD2 isotopomer. This kit has been tested with protein isotopic labeling in E. coli. It is beneficial for sample stability, which reduces sample heating because of high power decoupling when studying the side chain dynamics of proteins through solid-state NMR spectroscopy.

Conditionnement

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Code de la classe de stockage

11 - Combustible Solids

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

Articles

Methyl Isotope-Labeling of Proteins from NMR-Bio

Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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