861030

Auramine O

Dye content 85 %, certified by the Biological Stain Commission

• Synonyme(s) : 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum
• Formule empirique (notation de Hill): C₁₇H₂₁N₃ · HCl
• Numéro CAS: 2465-27-2
• Poids moléculaire : 303.83
• Numéro C.I. (Colour Index): 41000
• Numéro Beilstein : 4030061
• Numéro CE : 219-567-2
• Numéro MDL: MFCD00012484
• Code UNSPSC : 12171500
• ID de substance PubChem : 24888527
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: certified by the Biological Stain Commission
• Niveau de qualité: 200
• Forme: powder or chunks
• Composition: Dye content, 85%
• Technique(s): microbe id | staining: suitable
• Pf: >250 °C (dec.) (lit.)
• Solubilité: 95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow
• λmax: 370 nm; 434 nm (2nd)
• ε (coefficient d'extinction): ≥12000 at 249-255 nm in water at 0.005 g/L; ≥15000 at 367-373 nm in water at 0.005 g/L; ≥36000 at 429-435 nm in water at 0.005 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• Clé InChI: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.

Actions biochimiques/physiologiques

Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Adéquation

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H302,H319,H351,H411
• Conseils de prudence: P201 - P273 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3