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Key Documents

797006

Sigma-Aldrich

In Vitro Protein Expression (iPE-SS) Kit for disulfide-containing proteins

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.12

Utilisation

Stable Isotope Labeling
1 extractions

Niveau de qualité

Technique(s)

bio NMR: suitable
protein expression: suitable

Température de stockage

−70°C

Application

This is a kit which is improved for the synthesis of extracellular secretory proteins such as cytokines and antibodies. This kit is suitable for the synthesis of stable isotope labeled disulfide-containing proteins.
This kit has been developed under license from RIKEN, incorporating their proprietary, advanced cell-free protein synthesizing technology into a kit dedicated to stable isotope labeling.

Conditionnement

Stable isotope labeled amino acid mixtures are not included in this kit. Please use the separately sold stable isotope labeled amino acids mixtures. (Prod. No. 767972, 767964, and 771031)
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • Internal Solution-SS

  • External Solution-SS

  • Control DNA (pUC-BAP)

  • L-Glutathione oxidized disodium salt

  • L-Glutathione reduced

  • Dialysis Cup

Mentions de danger

Conseils de prudence

Classification des risques

Aquatic Chronic 3

Code de la classe de stockage

10 - Combustible liquids


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Consulter la Bibliothèque de documents

Jun Yokoyama et al.
Analytical biochemistry, 411(2), 223-229 (2011-01-25)
During recent years, the targets of protein structure analysis using nuclear magnetic resonance spectroscopy have become larger and more complicated. As a result, a complete and precise stable isotope labeling technique has been desired. A cell-free protein synthesis system is
Takayoshi Matsuda et al.
Biochemical and biophysical research communications, 431(2), 296-301 (2013-01-08)
Many important therapeutic targets are secreted proteins with multiple disulfide bonds, such as antibodies, cytokines, hormones, and proteases. The preparation of these proteins for structural and functional analyses using cell-based expression systems still suffers from several issues, such as inefficiency

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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