LC/MS Analysis of Anesthetics on Ascentis^® Express F5

column

Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 μm particles (53590-U)

mobile phase

[A] 10 mM ammonium acetate, pH 4.0 with acetic acid; [B] acetonitrile; (20:80, A:B)

flow rate

1.0 mL/min

pressure

2320 psi (160 bar)

column temp.

35 °C

detector

MS, ESI(+), SIR, m/z 235, 236, 247

injection

2 μL

sample

10 μg/mL in methanol