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HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections

HPLC Analysis Proteins on Proteomix® SCX-NP1.7, Demonstration of Column Stability with Repeated Injections application for HPLC

Materials

analytical column

Product No.
Description
Pricing

Proteomix® SCX-NP1.7 Strong Cation Exchange Guard Cartridge and Holder

L × I.D. 1 cm × 4 mm, 1.7 μm particle size, stainless steel

Proteomix® SCX-NP1.7 Strong Cation Exchange Column

L × I.D. 5 cm × 4.6 mm, 1.7 μm particle size, stainless steel

standard

Product No.
Description
Pricing

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Ribonuclease A from bovine pancreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

CONDITIONS

column

Proteomix SCX-NP1.7, 5 cm x 4.6 mm I.D., 1.7 μm particles (Z777146)

column

Proteomix SCX-NP1.7 guard cartridge with holder, 1 cm x 4 mm I.D. (Z777144)

mobile phase

(A) 20 mM sodium phosphate buffer, pH 6.5, (B) A + 1.0 M NaCl

gradient

0 - 100% B in 25 min, 15 min prewash

flow rate

0.5 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

each compound 1 mg/mL

Description

Legal Information

Proteomix is a registered trademark of Sepax Technologies