HPLC Analysis of Phenylacetic Acid on Ascentis^® C18

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] 20 mM phosphoric acid; [B] acetonitrile; (75:25, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 215 nm

injection

5 μL

sample

50 μg/mL in water: acetonitrile: methanol (75: 20: 5)