HPLC Analysis of Maté Leaves on Ascentis^® Express C18

sample/matrix

UAE (Ultrasonic Extraction) maté extract (dried and minced leaves (7.5 g) from maté were extracted with 225 mL of methanol in an ultrasonic bath, thermostated at 75 ± 0.5 °C (potency: 90 W; frequency: 40 kHz))

column

Ascentis Express C18, 150 x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] water, pH 3 with formic acid; [B] acetonitrile, pH 3 with formic acid

gradient

0 to 5% B in 20 min; 5 to 40% B in 30 min

flow rate

1 mL/min

column temp.

25 °C

detector

PDA: range 215- 420 nm (8 μL detector cell volume, cell temp. 40 °C, sampling rate 12.5 Hz, time constant 0.025 s)

injection

10 μL