HPLC Analysis of Glutethimide Enantiomers on Astec® Cellulose DMP
CONDITIONS
column
Astec Cellulose DMP, 25 cm x 4.6 mm I.D., 5 μm particles (51099AST)
mobile phase
[A] heptane; [B] isopropanol; (80:20, A:B)
flow rate
1 mL/min
column temp.
30 °C
detector
UV, 254 nm
injection
15 μL
sample
glutethimide, 1 g/L in heptane:isopropanol (50:50)
Description
Analysis Note
Glutethimide enantiomers are resolved by normal phase chromatography.
Other Notes
Glutethimide is used to treat insomnia.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany