HPLC Analysis of Chlorthalidone Enantiomers on Astec® CYCLOBOND I 2000 HP-RSP
Materials
analytical column
Product No.
Description
Pricing
Astec® CYCLOBOND I 2000 HP-RSP Chiral HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mmCONDITIONS
column
CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)
mobile phase
[A] 10 mM ammonium acetate, pH 4.0; [B] acetonitrile; (85:15, A:B)
flow rate
1 mL/min
column temp.
25 °C
detector
UV, 235 nm
injection
2 μL
sample
5 mg/mL in methanol
Description
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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