HPLC Analysis of Aflatoxins in Wheat on Ascentis® Express C18 after SPE using Supel™ Tox AflaZea
CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
25 g wheat meal spiked with pesticide standards
SPE tube/cartridge
Supel™Tox AflaZea, 6 mL (55314-U)
sample addition
2 mL
elution
collect eluate in 5 mL silanized culture tubes
eluate post-treatment
combine 200 μL of sample eluate and 880 μL of DI water in a 2 mL autosampler vial
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase
[A] water; [B] methanol; [C] acetonitrile; (720:120:120, A:B:C) containing 0.780 g/L potassium bromide, 230 μL/L nitric acid
flow rate
0.400 mL/min
pressure
4568 psi (315 bar)
column temp.
35 °C
detector
FLD 360 ex; 440 em
injection
20 μL
derivatization
via KOBRA electrochemical cell, 50 μA
Description
Analysis Note
Sample/matrix:
Weigh 25 grams of wheat meal into a 500 mL Erlenmeyer flask. Add 100 mL of 84:16 acetonitrile:water, seal, and place on an orbital shaker at med-high speed for 90 minutes. Filter the sample through a #2 qualitative filter. Spike sample at 2 mg/mL using analytical standards.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC