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A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

ABSTRACT

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Copper(II) bromide, 99.999% trace metals basis

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 53.5-56.5% in H₂O

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 40 wt. % in H₂O

Sigma-Aldrich

Copper(II) bromide, 99%

Sigma-Aldrich

2-Bromo-2-methylpropionic acid, 98%

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

Acetone, suitable for HPLC, ≥99.9%

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 1.0 M in methanol

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

Sigma-Aldrich

Acetone, Laboratory Reagent, ≥99.5%

Sigma-Aldrich

Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)

Sigma-Aldrich

Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

Sigma-Aldrich

Acetone, histological grade, ≥99.5%

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

Sigma-Aldrich

Deuterium oxide, filtered, 99.8 atom % D

Sigma-Aldrich

Dimethyl sulfoxide solution, 50 wt. % in H₂O

Sigma-Aldrich

Diethylamine, ≥99.5%

Sigma-Aldrich

L-Glutamine

Sigma-Aldrich

Deuterium oxide, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

Sigma-Aldrich

Deuterium oxide, 70 atom % D

Sigma-Aldrich

Deuterium oxide, 60 atom % D

Sigma-Aldrich

Dimethyl sulfoxide, ACS reagent, ≥99.9%

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water

Sigma-Aldrich

1,2-Dichloroethane, ACS reagent, ≥99.0%

Sigma-Aldrich

Dimethyl sulfoxide, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

Ethylenediamine, purified by redistillation, ≥99.5%

Sigma-Aldrich

N-Hydroxysuccinimide, 98%

SAFC

L-Glutamine