G005955
HPLC Analysis of HSA Fusion Protein on Zenix®-C SEC-300, Effect of Mobile Phase Composition
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
mobile phase: 150 mM sodium phosphate buffer, pH 7.0 or (A) 150 mM sodium phosphate buffer, pH 7.0; (B) IPA; (95:5, A:B)
flow rate: 1.0 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μL
sample: HSA fusion peptide 5 mg/mL (MW 75 kD, pl 5.0, HSA conjugated peptide in diabetes treatment)
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
analytical column
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