Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

G005862

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase: methanol
flow rate: 1.0 mL/min
pressure: 1653 psi (114 bar)
column temp.: 30 °C
detector: UV, 280 nm
injection: 5 μL
sample: 0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Analysis was conducted using standard solution, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard
    DL-alpha-Tocopherol acetate, analytical standard
    (±)-α-Tocopherol, analytical standard
    Phylloquinone (K1), analytical standard

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service