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G005814

GC Analysis of FAMEs in Atlantic Salmon on Omegawax

suitable for GC

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technique(s)

gas chromatography (GC): suitable

test parameters

column: Omegawax, 30 m x 0.25 mm I.D., 0.25μm (24136)
oven: 170 °C, 1 °C/min to 225°C
inj. temp.: 250 °C
detector: FID, 260 °C
carrier gas: helium, 1.2 mL/min
injection: 1 μL, 100:1 split
liner: 4 mm ID FocusLiner with taper
sample: 1. Fish Oil from capsule, prepared according to AOAC Method 991.39 2. Atlantic, farm-raised salmon, prepared according to AOCS Official Method Ce 1k-09

application(s)

food and beverages

Analysis Note

Samples of farm-raised Atlantic salmon were prepared and analyzed for fatty acids. The salmon was extracted using an acid digestion /alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09 (2). The resulting extracts were then analyzed by GC/FID on an Omegawax 250 capillary column as described in AOCS Official Method Ce 1i-07.

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analytical column

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