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Key Documents

G005710

LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express HILIC

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILlC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase: [A] 5 mM ammonium formate in water, pH 6.5; [B] 5 mM ammonium formate in acetonitrile, pH 6.5; (2:98, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 0.1 μL
sample: 1 mg/L each in mobile phase

suitability

application for HPLC

application(s)

clinical

Analysis Note

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of omeprazole metabolites.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Omeprazole sulfone
    Omeprazole
    5-Hydroxyomeprazole

analytical column

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Description
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standard

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