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G005677

HPLC Analysis of Beverage Additives in Diet Cola on Ascentis® Express C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] 20 mM ammonium acetate, pH 4.7 with acetic acid: [B] acetonitrile; (90:10, A:B)
flow rate: 0.6 mL/min
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 μL
sample: 100 - 500 μg/mL in 95:5, buffer: acetonitrile

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Benzoic acid
    Asp-Phe methyl ester

analytical column

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standard

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