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G005672

HPLC Analysis of Benzoate and Sorbate on Ascentis® Express RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)
mobile phase: [A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: UV, 215 nm
injection: 1 μL
sample: 50 mg/L each

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of sorbate and benzoate.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column

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