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G005588

HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide ("Green" Mobile Phase Conditions)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)
mobile phase: [A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)
flow rate: 0.4 mL/min
pressure: 138 bar (2000 psi) column, 248 bar (3600 psi) total
column temp.: 55 °C
detector: UV, 215 nm
injection: 1 μL
sample: 50 μg/mL in mobile phase

suitability

application for HPLC

application(s)

cleaning products
cosmetics
personal care

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Sorbic acid, analytical standard
    Benzoic acid, Standard for quantitative NMR, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

analytical column

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standard

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