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G005391

HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express RP-Amide

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 3 cm x 4.6 mm I.D., 2.7 μm particles (53921-U)
mobile phase: [A] 100 mM ammonium acetate, pH 5.6 with acetic acid; [B] water; [C] acetonitrile
gradient: 20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min
flow rate: 3 mL/min
pressure: 3336 psi (230 bar)
column temp.: 40 °C
detector: UV, 214 nm
injection: 1 mL/min
sample: diet soda spiked with neotame 350 μg/mL

suitability

application for HPLC

application(s)

food and beverages

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in diet cola.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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