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G004723

HPLC Analysis of t-BOC-Methionine Enantiomers on Astec® (R,R) P-CAP

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Astec (R,R) P-CAP, 25 cm x 4.6 mm I.D., 5 μm particles (31024AST)
mobile phase: 20 mM ammonium acetate in: (A) acetonitrile; (B) methanol; (70:30, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 220 nm
injection: 2 μL
sample: 5 mg/mL in methanol

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
P-CAP is a trademark of Sigma-Aldrich Co. LLC

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