G003710

HPLC Analysis of Nucleosides on Ascentis^® Phenyl

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Phenyl, 15 cm x 2.1 mm I.D., 5 μm particles (581613-U); mobile phase: 10 mM ammonium formate (pH 3.0 with formic acid); flow rate: 0.2 mL/min; column temp.: 35 °C; detector: UV, 270 nm; 750 psi back pressure regulator on outlet of flow cell; injection: 1 μL; sample: as indicated, in mobile phase
• suitability: application for HPLC
• application(s): life science and biopharma

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Cytidine, ≥99.0% (HPLC)
2′-Deoxycytidine hydrate, ≥99% (HPLC)
Guanosine, ≥98%
Uridine, ≥99%
Adenosine, ≥99%
3′-Deoxyguanosine .1 mg/mL
5′-Deoxyadenosine, methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase substrate .1 mg/mL
Thymidine, ≥99.0% (HPLC) .1 mg/mL
See All (8)