G001506A
HPLC Analysis of Carboxymethylated Apohemoglobin Tryptic Digest on Discovery® BIO Wide Pore C5
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)
mobile phase: [A] 0.1% TFA in water:0.1% TFA in acetonitrile, 95:5; [B] 0.1% TFA in water:0.1% TFA in acetonitrile; 50:50
gradient: 0-100% B in 65 min
flow rate: 1 mL/min
column temp.: 30 °C
detector: UV, 215 nm
injection: 50 μL
sample: carboxymethylated apohemoglobin tryptic digest in 50 mM ammonium bicarbonate
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
analytical column
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