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Key Documents

G001419

HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery HS C18, 15 cm × 4.6 mm I.D., 3 μm particles (569252-U)
mobile phase: 100% acetonitrile
flow rate: 0.8 mL/min
column temp.: 30 °C
detector: UV, 290 nm
injection: 10 μL
sample: 50 μg/mL each analyte

suitability

application for HPLC

application(s)

clinical
vitamins, nutraceuticals, and natural products

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description

    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard

analytical column

standard

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Description
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