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821841

TSKgel® ODS-100V (5 µm) HPLC Columns

L x I.D. 1 cm × 2 mm, C18 (octadecyl) phase

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About This Item

UNSPSC Code:
41115711

product name

TSKgel® ODS-100V Guard Cartridge, phase C18 (octadecyl), L × I.D. 1 cm × 2 mm, 5 μm particle size

material

stainless steel

Agency

suitable for USP L1

product line

TSKgel®

manufacturer/tradename

Tosoh 21841

extent of labeling

15% Carbon, endcapped with bifunctional dialkylsilane

parameter

0-100% organic solvent
10-50 °C temperature

technique(s)

HPLC: suitable

L × I.D.

1 cm × 2 mm

surface area

450 m2/g

matrix

spherical silica

matrix active group

C18 (octadecyl) phase

particle size

5 μm

operating pH

2.0-7.5

separation technique

reversed phase

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General description

Guard Cartridges for 4.6mmID TSKgel ODS-100V, 5um columns, 3pk TSKgel® Guard cartridges require a holder, which is sold separately. For 2 mm cartridges, the holder is Part No. 819308. For the 3.2 mm cartridges used to protect 4.6 mm columns, the holder is Part No. 819018.

The bonded phase in TSKgel ODS-100V columns is prepared by an incomplete first reaction with a difunctional octadecylsilane reagent, which is followed by endcapping with a mixture of two difunctional dialkylsilane reagents. Both reactions are performed under anhydrous conditions to promote the formation of a monomeric C18 bonded phase structure. The carbon content of TSKgel ODS-100V is 15%, while the carbon content of the fully bonded TSKgel ODS-100Z columns is 20%. Thus, while non-polar, the polarity of the ODS-100V stationary phase is high, which favors the retention of polar organic compounds. Thus, TSKgel ODS-100V columns can be used with mobile phases that do not contain organic solvent, making this column type very suitable for the analysis of very hydrophilic compounds.

Reversed-phase liquid chromatography (RPLC) is the preferred method for analysis and purification of polar and nonpolar compounds that are soluble in mixtures of water and organic solvents.

Physical form

Shipped in acetonitrile.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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