713-0854

GC Analysis of PCBs in Transformer Oil on SPB^®-5 after SPE using Supelclean^™ LC-Florisil^® and LC-Si

suitable for GC, application for SPE

Properties

• technique(s): gas chromatography (GC): suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: used transormer oil containing 50 ppb Aroclor 1254.; SPE tube/cartridge: Supelclean LC-Florisil, 1.0 g/6 mL (57057); SPE tube/cartridge: Supelclean LC-Si, 1.0 g/6 mL (57051); condition: 0.5 mL isooctane (Florisil tube); sample addition: 0.2 g transformer oil or 0.1 g waste oil; washing: 5 x 2 mL isooctane; column: SPB-5, 30 m × 0.32 mm I.D., 0.25 μm film (24048); oven: 40 °C (4 min) to 300 °C at 10 °C/min, hold 5 min; detector: ECD; carrier gas: nitrogen; injection: 1 μL, splitless (30 sec delay), then split (50:1)
• suitability: application for SPE
• application(s): environmental

Description

Application

Connect Supelclean LC-Si SPE tube (1 g/6 mL) in series to male luer outlet of Supelclean LC-Florisil SPE tube (1 g/6 mL) using an SPE Tube Adapter (57020-U). Apply up to 0.2 g transformer oil or 0.1 g waste oil to upper frit of Florisil SPE tube. Pass 5 x 2 mL isooctane through both Florisil and LC-Si SPE tubes. Oil interferences will be retained on SPE tubes whereas PCBs will elute with isooctane fractions. Collect and combine isooctane fractions using 10 mL volumetric flask. Analyze isooctane extract using GC-ECD.

Legal Information

Florisil is a registered trademark of U.S. Silica Company

SPB is a registered trademark of Merck KGaA, Darmstadt, Germany

Supelclean is a trademark of Sigma-Aldrich Co. LLC