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Supelco

SP®-2560 Capillary GC Column

L × I.D. 100 m × 0.25 mm, df 0.20 μm

Synonym(s):

SP-2560, 100M.20UM.25MM

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About This Item

UNSPSC Code:
41115710
eCl@ss:
32119290

material

fused silica

Quality Level

100

Agency

AOAC® 2012.13,Ce 1-h05,996.06
meets requirements for USP G5

parameter

≤25-250 °C temperature (isothermal or programmed)

Beta value

313

df

0.20 μm

technique(s)

gas chromatography (GC): suitable

L × I.D.

100 m × 0.25 mm

matrix active group

Non-bonded; poly(biscyanopropyl siloxane) phase

application(s)

agriculture
clinical
food and beverages

column type

capillary highly polar

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General description

Application: This highly polar biscyanopropyl column was specifically designed for detailed separation of geometricpositional (cis/trans) isomers of fatty acid methyl esters (FAMEs). It is extremely effective for FAME isomer applications.
USP Code: This column meets USP G5 requirements.
Phase:
  • Non-bonded
  • Poly(biscyanopropyl siloxane)
Temp. Limits:
  • Subambient to 250 °C (isothermal or programmed)

Other Notes

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Legal Information

AOAC is a registered trademark of AOAC International
SP is a registered trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
281880-06
281880-05
281880-01
281678-05
281678-04

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Fatty acid composition of human erythrocyte membranes by capillary gas chromatography-mass spectrometry.
Alexander LR
Journal of Chromatography. B, Biomedical Applications, 342, 1-12 (1985)
Evaluation of the CP-Sil 88 and SP-2560 GC columns used in the recently approved AOCS official method Ce 1h-05: Determination of cis-, trans-, saturated, monounsaturated, and polyunsaturated fatty acids in vegetable or non-ruminant animal oils and fats by capillary GLC method.
Ratnayake WMN
Journal of the American Oil Chemists' Society, 83 (6), 475-488 (2006)
Antonio Natalello et al.
Journal of agricultural and food chemistry, 67(16), 4472-4482 (2019-04-02)
This work investigated the effects of feeding whole pomegranate byproduct (WPB) to lambs on ruminal, liver, and intramuscular fatty acids (FA). Seventeen lambs, divided into two groups, were fed for 36 days with a cereal-based concentrate diet (CON) or with
B Schettino et al.
Journal of dairy science, 100(8), 6256-6265 (2017-06-12)
Chia seed (Salvia hispanica L.) is the greatest known plant source of n-3 α-linolenic acid. The present study evaluated the effects of 3 inclusion levels of chia seed [zero (control); low, 2.7% (CLow); and high, 5.5% (CHigh)] in diets of
Joseph D Donovan et al.
Journal of food science, 82(6), 1491-1499 (2017-05-10)
Butyrate possesses negative sensory qualities and is most effectively utilized in the intestine to provide energy to the colonocyte for the maintenance of intestinal health. Butyrate has also shown promise in the treatment of intestinal disorders and diseases such as
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Protocols

GC Analysis of a 37-Component FAME Mix on SP™-2560

GC Analysis of a 37-Component FAME Mix on SP™-2560

GC Analysis of cis/trans C18:1, C18:2, and C18:3 FAME Isomers on SP™-2560

Separation of null

AOAC Method 2012.13: GC Analysis of Fatty Acids in Powdered Infant Formula on SP™-2560

Separation of C8:0; C10:0; C11:0 (I.S.); C12:0; C13:0 (I.S.); C14:0; C14:1n5; C15:0; C16:0; C16:1n7; C17:0; C17:1n7; C18:0; C18:1 trans; C18:1 cis; C18:2n6; C18:2n7; C18:3n6 ; C18:3n3; C20:0; C20:1n9; C20:2n6; C20:3n3; C20:4n6; C22:0; C22:1n9; C22:6n3 (DHA); C24:0

GC Analysis of a 37-Component FAME Mix on SP™-2560, Slower Oven Ramp

Separation of null

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Chromatograms

suitable for GCsuitable for GCsuitable for GCsuitable for GC, application for SPE

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