ZRB1739

Anti-LMBR1L Antibody, clone 1D6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Protein LMBR1L;Limb region 1 protein homolog-like;Lipocalin-1-interacting membrane receptor;LIMR
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D6, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 55.21 kDa; observed mol wt ~58 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_060583
• UniProt accession no.: Q6UX01
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... LMBR1L(55716)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects human LMBR1L. It targets an epitope within 16 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human LMBR1L.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Human LMBR1L.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human LMBR1L.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound LMBR1L peptide with a KD of 5.8 x 10-8 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected recombinant Human LMBR1L.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting with recombinant Human LMBR1L.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Human LMBR1L.

Target description

Protein LMBR1L (UniProt: Q6UX01; also known as Limb region 1 protein homolog-like, Lipocalin-1-interacting membrane receptor, LIMR) is encoded by the LMBR1L (also known as KIAA1174, LIMR, UNQ458/PRO783) gene (Gene ID: 55716) in human. LIMBR1L is a multi-pass membrane protein with five extracellular domains, nine transmembrane domains, and five cytoplasmic domains. Its N-terminal region contains a lipocalin binding site (aa 1-76). It is shown to be highly specific for lipocalin 1 (Lcn1) and binds to Lcn1 with low micromolar to high nanomolar affinity and mediates its endocytosis. It is widely expressed in human tissues and plays an essential role in lymphocyte development by negatively regulating the canonical Wnt signaling pathway. It stabilizes the β-catenin destruction complex that is required for regulating catenin β1 levels. In murine models, the absence of LMBR1L is shown to cause aberrant activation of Wnt/β-catenin signaling, which leads to impaired development and functional defects in all lymphoid lineages. LMBR1L-deficient T cells are predisposed to apoptosis and die in response to antigen-specific expansion signals. Five isoforms of LMBR1L have been described that are produced by alternative splicing. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Heselink, RW., and Findlay, JBC. (2013). Mol. Membr. Biol. 30(5/6); 327-337; Wojnar, P., et al. (2001). J. Biol. Chem. 276(23); 20206-20212).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable