ZRB1558
Anti-eIF2A Antibody, clone 2C18 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Eukaryotic translation initiation factor 2A;65 kDa eukaryotic translation initiation factor 2A;eIF-2A
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C18, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 64.99 kDa
observed mol wt ~60 kDa
purified by
using Protein A
species reactivity
human, mouse, rat
species reactivity (predicted by homology)
monkey, canine
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2C18 is a Rabbit recombinant monoclonal antibody that specifically detects Eukaryotic translation initiation factor 2A (eIF-2A). It targets an epitope within 23 amino acids from the C-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human Eukaryotic translation initiation factor 2A (eIF-2A).
Application
Quality Control Testing
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF2A in PC12 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF2A in mouse pancreas tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF2A in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF2A in U2OS cells..
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected eIF2A in PC12 cell lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected eIF2A in mouse pancreas tissue lysate.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected eIF2A in human pancreas tissue sections.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected eIF2A in U2OS cells..
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Eukaryotic translation initiation factor 2A (UniProt: Q9BY44; also known as eIF-2A, 65 kDa eukaryotic translation initiation factor 2A) is encoded by the EIF2A (also known as CDA02, MSTP004, MSTP089) gene (Gene ID: 83939) in human. eIF-2A is a widely expressed protein with higher levels observed in pancreas, heart, brain, and placenta. It functions in the early steps of protein synthesis by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. It is reported to catalyze the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low magnesium levels. It has seven WD repeats. Although both eIF2 and eIF-2A function in the methionyl-puromycin assays, the order of events differs between the two. In contrast to the eIF-2 complex, it binds methionyl-tRNAi to 40S subunits in a codon-dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner. Note: The gene EIF2A should not be confused with EIF2S1 (Gene ID: 1965) that is frequently referred to as eIF2-alpha in the literature. EIF2S1 is the alpha subunit of the eIF2 translation initiator complex. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Zoll, WL., et al. (2002). J. Biol. Chem. 277(40); 37079-37087).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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