ZRB1327
Anti-Centrin-1 Antibody, clone 5L17 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Sign Into View Organizational & Contract Pricing
All Photos(10)
About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
5L17, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 19.57 kDa
observed mol wt ~18 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminus
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 5L17 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Centrin-1. It targets an epitope within 21 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal region of human Centrin-1.
Application
Quality Control Testing
Evaluated by Western Blotting in Human testis tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Centrin-1 in Human testis tissue lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Centrin-1 in F9, NTERA2, and HeLa cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Centrin-1 in human testis, human kidney, and human lung tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Centrin-1 with a KD of 2.9 x 10-9.in an Affinity Binding Assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Human testis tissue lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Centrin-1 in Human testis tissue lysate.
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Centrin-1 in F9, NTERA2, and HeLa cell lysates.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Centrin-1 in human testis, human kidney, and human lung tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Centrin-1 with a KD of 2.9 x 10-9.in an Affinity Binding Assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Centrin-1, clone 5L17 ZooMAb®, Cat. No. ZRB1327, is a recombinant Rabbit monoclonal antibody that specifically targets Centrin-1 and is tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.
Target description
Centrin-1 (UniProt: Q12798; also known as Caltractin isoform 2) is encoded by the CETN1 (also known as CEN1, CETN) gene (Gene ID: 1068) in human. Centrins are calcium-binding phosphoproteins with four Ca2+-binding EF-hand domains that are localized to the centrosome of all eukaryotes. Three separate Centrin genes, CETN1-3, have been identified in both mouse and human. Centrin-1 is a monomeric protein that is expressed in testis tissue and in photoreceptor cells. Its expression has not been reported in any other healthy tissue. It plays a fundamental role in microtubule-organizing center structure and function and has a role in sperm cilia formation. A significantly higher expression of Centrin-1 has been reported in pancreatic and prostate cancers and CETN1 knockdown inhibits cell proliferation in breast cancer. Hence, it has been classified as cancer/testis antigen. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Jiao, R., et al. (2019). Cancer Med. 8(11); 5289-5300; Kim, J., et al. (2013). Biomarker Res. 1; Article number: 22).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service