ZRB1023
Anti-Laminin α2 Antibody, clone 2I22 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2I22, recombinant monoclonal
monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 343.91 kDa
observed mol wt ~350 kDa
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... LAMA2(3908)
mouse ... Lama2(16773)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 2I22 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Laminin subunit alpha-2. It targets an epitope within 18 amino acids from the N-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Laminin 2 alpha.
Application
Anti-Laminin a2, clone 2I22 ZooMAb, Cat. No. ZRB1023, is a recombinant Rabbit monoclonal antibody that specifically targets Laminin 2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blo
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Laminin 2 in human heart tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound Laminin 2a peptide with a KD of 1.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin 2 in AC16 and C2C12 cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Laminin α2 in human heart tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Affinity Binding Assay: A representative lot of this antibody bound Laminin 2a peptide with a KD of 1.0 x 10-8 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin 2 in AC16 and C2C12 cells.
Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Laminin α2 in human heart tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Laminin a2 (UniProt: P24043; also known as Laminin M chain, Laminin-12 subunit alpha, Laminin-2 subunit alpha, Laminin-4 subunit alpha, Merosin heavy chain) is encoded by the LAMA2 (also known as LAMM) gene (Gene ID: 3908) in human. Laminins are a family of heterotrimeric extracellular glycoproteins consisting of alpha, beta, and gamma chains that are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Laminins mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Thus far five alpha, three beta, and three gamma subunits are known, which assemble into at least 15 different laminin molecules. Laminin a2 is a glycoprotein with multiple EGF-like domains and is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off. It is expressed in multiple tissues, including placenta, striated muscle, cardiac muscle, pancreas, lung, spleen, skin, bone, and some regions of the brain. Laminin a2 binds to cells via a high affinity receptor and mediates the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Mutations in LAMA2 gene have been linked Merosin-deficient congenital muscular dystrophy 1A (MDC1A) and limb-girdle muscular dystrophy that results in muscle weakness and atrophy. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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