ZMS1103

Anti-Poly(ADP-ribose) Antibody, clone 10H ZooMAb^® Mouse Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): PAR, Poly (ADP-ribose) polymer
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 10H, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 559.32 kDa; observed mol wt ~200 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): all
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 10H a ZooMAb^® mouse recombinant monoclonal antibody that specifically detect Poly (ADP-Ribose) polymer.

Immunogen

Poly(ADP-Ribose) polymer mixed with methylated bovine serum albumin.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Poly(ADP-ribose) in HeLa cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Poly(ADP-ribose) in HeLa treated with 0.1% Hydrogen Peroxide, but not in untreated cells.

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated Poly(ADP-ribose) in HeLa treated with 0.1% Hydrogen Peroxide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Poly(ADP-ribose) (PAR) that is synthesized from NAD+ by PAR polymerases (PARPs) is reported to regulate many physiological processes such as the maintenance of DNA integrity, gene expression and cell division. It is a unique post-translational modification that regulates cell survival and cell-death. This modification is brought about by the action of poly(ADP-ribose) polymerase (PARP), an abundant nuclear enzyme that uses nicotinamide adenine dinucleotide (NAD+). In humans, 17 different PARPs have been described, six of these add multiple ADP-ribose units and nine add only single units. Poly(ADP-ribose) contains a DNA-binding domain with two zinc fingers in its N-terminal domain, which is linked to the C-terminal NAD+-binding domain by a short region containing several glutamic acid residues that are sites of auto-poly(ADP-ribosyl)ation). Synthesis of poly(ADP-ribose) within the cell is initiated by agents that generate DNA strand interruptions. The branched homopolymer chains can reach a length of 200 to 300 residues but they are rapidly degraded after synthesis. Poly(ADP-ribose) are degraded by two classes of enzymes. The first enzyme cleaves ribose-ribose bonds between ADP-ribose subunits and the second enzyme removes the terminal ADP-ribose group from modified proteins. Poly(ADP-ribose) glycohydrolase (PARG) is the primary enzyme that cleaves ribose-ribose bonds. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Leung, AKL. (2014). J. Cell Biol. 205(5); 613-619; Schreiber, V., et al. (2006). Nat. Rev. Mol. Cell Biol. 7(7); 517-528).

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable