ZMS1088
Anti-MGMT Antibody, clone MT3.1 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Methylated-DNA-protein-cysteine methyltransferase;ECC:2.1.1.63;6-O-methylguanine-DNA methyltransferase;O-6-methylguanine-DNA-alkyltransferase
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
mouse
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
MT3.1, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 21.65 kDa
observed mol wt ~22 kDa
purified by
using protein G
species reactivity
human
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MGMT(4255)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone MT3.1 is a ZooMAb® Mouse recombinant monoclonal antibody that specifically detects human Methylated-DNA-protein-cysteine methyltransferase (MGMT).
Immunogen
His-tagged, full-length recombinant human Methylated-DNA-protein-cysteine methyltransferase (MGMT).
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell nuclear extract
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MGMT in Raji cell nuclear extract.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MGMT in lysate from MCF-7 cells and nuclear extract from Jurkat cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MGMT in MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MGMT in human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Raji cell nuclear extract
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MGMT in Raji cell nuclear extract.
Evaluated by Western Blotting in Raji cell nuclear extract
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MGMT in Raji cell nuclear extract.
Tested applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MGMT in lysate from MCF-7 cells and nuclear extract from Jurkat cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MGMT in MCF-7 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MGMT in human small intestine tissue sections.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Raji cell nuclear extract
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MGMT in Raji cell nuclear extract.
Target description
Methylated-DNA-protein-cysteine methyltransferase (UniProt: P16455; also known as EC:2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID: 4255) in human. MGMT is a ubiquitous DNA repair suicide enzyme that removes the cytotoxic DNA adducts O6-methylguanine and O6-chloroethylguanine induced by methylating and chloroethylating agents. It repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. Hence, it serves as a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. MGMT is reported to be frequently silenced due to abnormal methylation in cancer cells. However, it also makes tumor cells more sensitive to radiation therapy and certain alkylating drugs. In adults, epigenetic silencing of MGMT by promoter methylation has been shown to improve the outcome in subjects with glioblastoma multiforme receiving temozolomide, a methylating agent. Higher activity of MGMT in cancer cells is reported to be one of the major mechanisms of cancer drug resistance that which counteracts chemotherapy-induced DNA alkylation. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Fan, C-H., et al. (2013). Cell Death Dis. 4; e876).
Physical form
Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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