Y2627
2xYT Medium
EZMix Powder microbial growth medium
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
Recommended Products
grade
for molecular biology
description
nutrient rich, quick dissolve, dust free formulation
sterility
non-sterile
form
powder
composition
Inert binder (EZMix only), 0.6 g/L
NaCl, 5 g/L
Tryptone (pancreatic digest of casein), 16 g/L
Yeast extract, 10 g/L
application(s)
agriculture
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
2xYT EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Easy scale-up using larger package size
- Standard formulation
Reconstitution
Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Legal Information
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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