U6504

Urea

suitable for electrophoresis

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352111
• PubChem Substance ID: 24900435
• NACRES: NB.22

Properties

• Assay: ≥98%
• Quality Level: 200
• form: powder or crystals
• technique(s): electrophoresis: suitable
• impurities: ≤0.005% Ammonia
• mp: 132-135 °C (lit.)
• solubility: H₂O: 100 mg/mL
• density: 1.335 g/mL at 25 °C (lit.)
• cation traces: Fe: ≤5 ppm; Pb: ≤5 ppm
• absorption: ≤0.06 at 260 at 5 M; ≤0.06 at 280 at 5 M
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759) , CA2(760) ; rat ... Ppm1a(24666)

Description

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves