T6632

Thymidine Phosphorylase, recombinant from Escherichia coli

recombinant, expressed in E. coli, buffered aqueous solution, ≥900 units/mL, 0.2 μm filtered

• Synonym(s): Thymidine:orthophosphate deoxy-D-ribosyltransferase
• CAS Number: 9030-23-3
• Enzyme Commission number: 2.4.2.4 (BRENDA, IUBMB)
• MDL number: MFCD00132426
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• recombinant: expressed in E. coli
• Quality Level: 300
• sterility: 0.2 μm filtered
• form: buffered aqueous solution
• concentration: ≥900 units/mL
• UniProt accession no.: P07650
• storage temp.: 2-8°C
• Gene Information: Escherichia coli K12 ... deoA(948901)

Description

General description

Thymidine phosphorylase inhibits vascular smooth muscle cell proliferation.

Application

Thymidine phosphorylase has been used in a study to evaluate biomarkers for advanced breast cancer patients treated with capecitabine-based first-line chemotherapy. Thymidine phosphorylase has also been used in a study to investigate implications for the clinical efficacy of nucleoside analogues.

Biochem/physiol Actions

An enzyme that catalyzes the reversible conversion of thymidine to thymine. Thymidine phosphorylase is part of the pyrimidine nucleoside salvage pathway. This pathway allows pyrimidine bases to be recycled for nucleotide biosynthesis, while the pentose 1-phosphates are converted to intermediates of the pentose phosphate shunt and glycolysis. The E. coli thymidine phosphorylase shares 40% sequence homology with the human sequence, which has been found to be identical to the angiogenic agent platelet-derived endothelial growth factor. The purified E. coli enzyme has been shown to stimulate blood vessel growth in chick chorioallantoic membrane assays.

Unit Definition

One unit will convert 1.0 μmole each of thymidine and phosphate to thymine and 2-deoxyribose 1-phosphate per min at pH 7.4 at 25°C.

Physical form

Solution in 0.5 M potassium phosphate containing 2 mM uracil, 0.02% sodium azide and bovine serum albumin

Preparation Note

Cloned from E. coli and produced in overexpressing E. coli

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves