SRP8034

IL-2 (human): FC (human)

recombinant, expressed in HEK 293 cells, >95% (SDS-PAGE)

• Synonym(s): Aldesleukin, INN, Interleukin-2, T-cell growth factor
• CAS Number: 110942-02-4
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• Assay: >95% (SDS-PAGE)
• form: lyophilized
• mol wt: ~42 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: <0.01 EU/μg endotoxin, tested
• color: white
• UniProt accession no.: P60568
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL2(3558)

Description

General description

Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors. IL-2 Superkine (Fc) is an artificial variant of IL-2 containing mutations at positions L80F / R81D / L85V / I 86V / I92F. These mutations are located in the molecule′s core that acts to stabilize the structure and to give it a receptor-binding conformation mimicking native IL-2 bound to CD25. These mutations effectively eliminate the functional requirement of IL-2 for CD25 expression and elicit proliferation of T cells. Compared to IL-2, the IL-2 superkine induces superior expansion of cytotoxic T cells, leading to improved antitumour responses in vivo, and elicits proportionally less toxicity by lowering the expansion of Tregulatory cells and reducing pulmonary oedema.

Physical form

Lyophilized from PBS.

Reconstitution

Reconstitute with 100 μL deionized water.

Other Notes

The extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) is fused at the C-terminus to the Fc portion of human IgG2.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable