SRP6522

Ubiquitin-TAMRA-labeled (TMR-Ub)

≥95% (SDS-PAGE)

• Synonym(s): CEP80, HUBCEP80, RPS27A, Ribosomal Protein S27a, UBA80, UBB, UBCEP1, UBCEP80
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• Assay: ≥95% (SDS-PAGE)
• form: liquid
• mol wt: 9.133 kDa
• packaging: pkg of 50 μg
• concentration: 8 mg/mL
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Post-translational modification of proteins by ubiquitin (Ub) is a key regulatory process that impacts almost all cellular functions. Ubiquitylation occurs through isopeptide linkage between the C-terminus of Ub and the e-amino group of a lysine (Lys) residue on the target substrate [1]. Ub itself has seven Lys residues (6, 11, 27, 29, 33, 48, and 63), any of which can participate in further ubiquitylation, generating polyUb chains [2, 3]. Monitoring the ubiquitylation of target proteins or the growth of polyubiquitin chains has traditionally been carried out with either radiolabeled or epitope-tagged ubiquitin requiring long and laborious detection methods. Fluorescently labeled ubiquitin provides a rapid, facile technique for studying ubiquitin conjugation in vitro. Unlike others, TheTAMRA-labeled ubiquitin carries a single TAMRA molecule attached at a defined location and avoids modification of either the N-terminus or Lys side chains.

Physical form

≥ 8 mg/mL in PBS.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable