SRP6454

Decorin/Bone proteoglycan II human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): CSCD, DCN, DSPG2, PG40, PGII, PGS2, SLRR1B
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized
• mol wt: calculated mol wt 38.8 kDa; observed mol wt 45 kDa (DTT-reduced. Protein migrates due to glycosylation. Gly 17 is the predicted N-terminal.)
• packaging: pkg of 20 and 100 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P07585
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... DCN(1634)

Description

General description

Decorin, also known as bone proteoglycan II, PGS2, SLRR1B, DCN, DSPG2 and PG40, is a secreted chondroitin /dermatan sulfate proteoglycan in the family of small leucine-rich proteoglycans (SLRPs). Decorin is a small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. Decorin and biglycan are thought to be the result of gene duplication. This protein is a component of connective tissue, binds to type I collagen fibrils, and plays a role in matrix assembly. Decorin appears to influence fibrillogenesis, and also interacts with fibronectin, thrombospondin, the complement component C1q, epidermal growth factor receptor (EGFR) and transforming growth factor-β (TGF-β). Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD).

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable