SRP2078

p53 (1-342) C-terminal deletion human

recombinant, expressed in insect cells, ≥80% (SDS-PAGE)

• Synonym(s): FLJ92943, LFS1, P53, TRP53
• UNSPSC Code: 12352200
• NACRES: NA.26

Properties

• biological source: human
• recombinant: expressed in insect cells
• Assay: ≥80% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~39.5 kDa
• packaging: pkg of 5 μg
• concentration: 250 μg/mL
• color: clear colorless
• NCBI accession no.: NM_000546
• UniProt accession no.: P04637
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... TP53(7157)

Description

Biochem/physiol Actions

Human p53 protein is composed of 393 amino acid residues with several distinct regions. The N-terminal activation domain allows p53 protein to recruit the basal transcription machinery and activate the expression of target genes. The core domain binds to target DNA in a sequence-specific manner and the majority of mutations found in human tumors occur in the region of the gene encoding this domain. The C-terminal domain is composed of predominantly basic residues and modification of the C-terminal basic domain, including acetylation, glycosylation and phosphorylation, is an essential mechanism for regulating p53 function. Disruption or loss of oligomerization function is associated with loss of cell cycle arrest. This mutant protein (with the deletion of the C-terminus 51 residues including the entire basic domain and a portion of the tetramerization domain) can be used as a unique tool to study specific functions of p53 related to the C-terminus.

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable