SRP0729
PCTK3 (CDK18)/CYCLINY, Active
PRECISIO® Kinase, recombinant
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.77
recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
35-55 nmol/min·mg (radiometric)
mol wt
~64 kDa (Cyclin Y)
~76 kDa (PCTK3)
concentration
0.1 μg/μL
accession no.
BC011526
NM_145012
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCNY(219771) , CDK18(5129)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Full-length recombinant human PCTK3 (CDK18) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2 (1). PCTK3 is expressed in a variety of human cell lines and tissues (2). Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer′s disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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