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recombinant
expressed in baculovirus infected Sf9 cells
Assay
70-95% (SDS-PAGE)
form
frozen liquid
specific activity
4.0-6.0 nmol/min·mg (radiometric)
mol wt
~74 kDa
concentration
0.1 μg/μL
accession no.
NM_007174
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... STK21(11113)
Application
Useful for kinase assay and Western blot.
Biochem/physiol Actions
Recombinant human STK21 (CIT) (1-449) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK21 is a serine/threonine-protein kinase that functions in cell division and also to promote efficient cytokinesis. STK21 is a novel molecular mechanism for a subset of human malformation syndromes of the central nervous system (1). STK21 is also associated with bipolar disorder and risk for schizophrenia. STK21 is a novel partner for the GTP-bound forms of rho and rac (2).
Reconstitution
Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.
Storage and Stability
Store in aliquots at -70°C. Avoid freeze/thaw cycles.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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