SRP0169

JMJD2B Active human

recombinant, expressed in baculovirus infected insect cells, ≥40% (SDS-PAGE)

• Synonym(s): JHDM3B, JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, KIAA0876, Lysine-specific demethylase 4B (KDM4B)
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥40% (SDS-PAGE)
• form: aqueous solution
• mol wt: 57 kDa
• packaging: pkg of 100 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_015015
• UniProt accession no.: O94953
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... KDM4B(23030)

Description

General description

Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No.NM_015015) (amino acids 1-500) with C-terminal FLAG tag, MW = 57 kDa, expressed in Sf9 cells using a Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme required to demethylate 1 pmol of substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.