SAE0110

HRV-3C Protease, Biotin tagged

Recombinant protein, 0.8-1.2 mg/mL, aqueous solution

• Synonym(s): Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
• UNSPSC Code: 12352202
• NACRES: NA.54

Properties

• biological source: human (human Rhinovirus Type 14)
• recombinant: expressed in E. coli
• Assay: ≥90%
• form: aqueous solution
• specific activity: ≥5000 U/mg
• mol wt: 22 kDa
• concentration: 0.8-1.2 mg/mL
• technique(s): protein purification: suitable
• suitability: suitable for protein modification
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.This sequence is: Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro.Proteolytic cleavage occurs between the Gln and Gly residues. The HRV-3C protease is useful for cleaving recombinant proteins that are expressed as fusion proteins with this sequence between the carrier domain and the protein of interest.
This biotinylated HRV-3C protease is intended for on-column cleavage of fusion proteins with an HRV-3C cleavage site. It specifically cleaves the protein of interest from a column-bound fusion protein, leaving the fusion domain or tag bound to the affinity column (e.g. Ni-NTA column) and eluting only the protein of interest. This method is advantageous over post-elution cleavage for several reasons:

• It eliminates most impurities normally associated with purification on Ni-chelating columns.
• It allows gentler elution conditions, with added flexibility in the elution buffer composition. This can mitigate protein aggregation and inactivation.

After cleavage, the protease can be removed with any avidin-conjugated or streptavidin-conjugated beads. This product has been enzymatically biotinylated with no effect on its proteolytic activity. It has no additional protein purification tags. The product is supplied in aqueous buffer (0.8–1.2 mg/mL) with 20 mM Trizma^®-HCl, pH 8.0, 200 mM NaCl, 1 mM TCEP, and 50% (v/v) glycerol.

Preparation Note

The product is supplied in aqueous buffer (0.8–1.2 mg/mL) containing 20 mM Trizma^®-HCl, pH 8.0, 200 mM NaCl, 1 mM TCEP, and 50% (v/v) glycerol.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable