SAE0023

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

• Synonym(s): Alanine Racemase from Bacillus stearothermophilus
• CAS Number: 9024-06-0
• Enzyme Commission number: 5.1.1.1 (BRENDA, IUBMB)
• MDL number: MFCD03454300
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Bacillus sp. (B. stearothermophilus)
• Quality Level: 200
• form: buffered aqueous solution
• specific activity: ≥10 IU/mg solid
• mol wt: M_r 78 kDa (2 subunits 39 kDa each)
• storage temp.: −20°C

Description

Application

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-¹⁴C]alanine to a racemic mixture of L/D- [¹⁴C]alanine †.

Biochem/physiol Actions

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

Unit Definition

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

Physical form

Aqueous solution containing 10 mM potassium phosphate

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable