SAE0021
Pyruvate Kinase M2 human
recombinant, expressed in E. coli, specific activity ≥100 unit/mg protein
Synonym(s):
Cytosolic Thyroid hormone-binding protein (CTHBP), M2-PK, OPA-interacting protein 3 (OIP-3), PKM2, Pyruvate kinase 3 (PK3), Pyruvate kinase muscle isozyme, p58
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
lyophilized powder
specific activity
≥100 units/mg protein
shipped in
ambient
storage temp.
−20°C
General description
Research area: Cell signaling This product is recombinant human PKM2 (P14618) expressed in E. coli and has a predicted molecular mass of 58 kDa. The embryonic pyruvate kinase M2 (PKM2) is one of the isoforms of pyruvate kinases (PK), found in mammals. It is coded by the PKM2 gene mapped to human chromosome 15q23. PKM2 is expressed in all cells excluding adult muscle, brain, and liver. This protein is usually present, as an active tetrameric form.
Application
Human pyruvate kinase M2 (PKM2) has been used to study the effects of phosphorylated PKM2 in inactivating myofibroblasts during renal fibrosis.PyruvateKinase M2 human has been used as an enzyme to study the inhibitory effect of a novel irreversible inhibitor on the kinaseactivity of PKM2. It has also been used in recombinant protein labelingand in pyruvate kinase enzyme activity assay.
Biochem/physiol Actions
Pyruvate kinase (PK) is an important glycolytic enzyme catalyzing the last step of glycolysis, transferring a phosphate group from phosphoenolpyruvate to ADP to yield one molecule of ATP and one molecule of pyruvate. The embryonic pyruvate kinase M2 (PKM2) controls β-catenin transactivation. It plays an important role in aerobic glycolysis or the Warburg effect. PKM2 induces gene transcription and tumorigenesis.Pyruvate Kinase M2 (PKM2)plays a role in the regulation of cell growth, cell proliferation, andapoptosis. It is found predominantly in embryonic tissues and several humancancers. PKM2 acts as a cytosolic thyroid hormone binding protein(CTHBP) and as a phosphotyrosine binding protein. PKM2 binds tophosphotyrosine peptides which results in the release of its allostericactivator fructose-1,6-bisphosphate (FBP) is associated with the blockageof PKM2 activity.
Unit Definition
One unit will convert 1.0 μmole of phosphoenol-pyruvate to pyruvate per minute at pH 7.6 at 37 °C, in the presence of 1 mM fructose-1,6-bisphosphate.
Physical form
Supplied as a lyophilized powder containing phosphate buffer at pH7.5, NaCl, DTT and a carbohydrate stabilizer.
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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PKM2 Phosphorylates Histone H3 and Promotes Gene Transcription and Tumorigenesis.
Yang W, et al.
Cell, 150(4), 685-696 (2012)
Pyruvate kinase isoenzyme M2 expression correlates with survival of cardiomyocytes after allogeneic rat heterotopic heart transplantation.
Shi J, et al.
Pathology Research and Practice, 211(1), 12-19 (2015)
Inhibiting aerobic glycolysis suppresses renal interstitial fibroblast activation and renal fibrosis
Ding H, et al.
American Journal of Physiology: Renal Physiology, 313(3), F561-F575 (2017)
Gilles A Spoden et al.
Experimental cell research, 315(16), 2765-2774 (2009-07-01)
The glycolytic key regulator pyruvate kinase M2 (M2-PK or PKM2) can switch between a highly active tetrameric and an inactive dimeric form. The transition between the two conformations regulates the glycolytic flux in tumor cells. We developed specific M2-PK-binding peptide
Hao Ding et al.
American journal of physiology. Renal physiology, 313(3), F561-F575 (2017-02-24)
Chronic kidney diseases generally lead to renal fibrosis. Despite great progress having been made in identifying molecular mediators of fibrosis, the mechanism that governs renal fibrosis remains unclear, and so far no effective therapeutic antifibrosis strategy is available. Here we
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