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Key Documents

SAB4700572

Sigma-Aldrich

Monoclonal Anti-CD54 low endotoxin antibody produced in rat

clone YN1/1.7.4, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Icam1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rat

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

YN1/1.7.4, monoclonal

form

buffered aqueous solution

species reactivity

mouse

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Icam1(15894)

Related Categories

General description

The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Immunogen

Mouse NS-1 cells

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 1 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Zhengyu Ma et al.
PLoS biology, 6(2), e43-e43 (2008-02-29)
At the interface between T cell and antigen-presenting cell (APC), peptide antigen presented by MHC (pMHC) binds to the T cell receptor (TCR) and initiates signaling. The mechanism of TCR signal initiation, or triggering, remains unclear. An interesting aspect of

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