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RAB0420

Sigma-Aldrich

Human Serum Amyloid A ELISA Kit

for serum, plasma, cell culture supernatant and urine

Synonym(s):

SAA, Serum amyloid A-1 protein 

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type urine
sample type plasma
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.41-300 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SAA1(6288)

General description

Serum amyloid A1 (SAA1) is an acute response protein. It is synthesized by the liver, during infection. SAA1 is expressed in the amnion and chorion of human fetal membranes. It is located on human chromosome chromosome 11. SAA1 encodes for the inducible forms of serum amyloid A (SAA) proteins.
The Human SAA (Serum Amyloid A) ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SAA in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human SAA

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Serum amyloid A1 (SAA1) induces the generation of proinflammatory cytokines like, IL-1β (interleukin 1 β), interleukin 6 (IL-6) and the uterotonic hormone proinflammatory cytokines and prostaglandin E2 (PGE2).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The human serum amyloid A protein (SAA) superfamily gene cluster: mapping to chromosome 11p15. 1 by physical and genetic linkage analysis
Sellar G C, et al.
Genomics, 19(2), 221-227 (1994)
Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
Li W, et al.
Scientific reports, 7(1), 693-693 (2017)
Melissa G Works et al.
Journal of immunology (Baltimore, Md. : 1950), 193(7), 3278-3287 (2014-08-27)
Psoriasis is a chronic autoimmune disease affecting the skin and characterized by aberrant keratinocyte proliferation and function. Immune cells infiltrate the skin and release proinflammatory cytokines that play important roles in psoriasis. The Th17 network, including IL-23 and IL-22, has

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