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RAB0325

Sigma-Aldrich

Inhibin-B EIA Kit

for serum, plasma, culture supernatant and cell lysates

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About This Item

UNSPSC Code:
41123300
NACRES:
NA.32

species reactivity

rat, mouse, human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
enzyme immunoassay: suitable

input

sample type plasma
sample type cell lysate
sample type serum
sample type culture supernatant(s)

assay range

inter-assay cv: <15%
intra-assay cv: <10%
sensitivity: 2 pg/mL
standard curve range: 1-10000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... INHBB(3625)

General description

Inhibin-B (INHBB) is a heterodimeric glycoprotein with disulfide-linkage. It is produced by granulosa cells in the ovary. The INHBB gene is mapped to human chromosome 2q14.2. Inhibin-B inhibits follicle-stimulating hormone (FSH) secretion and synthesis. It may serve in diagnosing testicular function and as an indicator of Sertoli cell numbers and function. Inhibin B may serve as a marker for spermatogenesis.
The Inhibin B Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Inhibin B peptide based on the principle of Competitive Enzyme Immunoassay. EIA-INB-1 targets the β B subunit, and therefore theoretically detects Activin B and Activin AB in addition to Inhibin B.
The Inhibin B Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting Inhibin B peptide based on the principle of Competitive Enzyme Immunoassay. EIA-INB-1 targets the β B subunit, and therefore theoretically detects Activin B and Activin AB in addition to Inhibin B.

Immunogen

Inhibin-B, synthetic peptide

Application

Inhibin-B EIA Kit has been used in quantification of Inhibin-B using enzyme-linked immunosorbent assay (ELISA) in Zika virus-infected human Sertoli cells (HSerC) and 8-week-old mice.

Kit Components Also Available Separately

Product No.
Description
SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S J Meachem et al.
European journal of endocrinology, 145(5), 561-571 (2001-11-27)
The recent availability of specific inhibin assays has demonstrated that inhibin B is the relevant circulating inhibin form in the human male. Inhibin B is a dimer of an alpha and a betaB subunit. It is produced exclusively by the
Mahamud-Ur Rashid et al.
PLoS neglected tropical diseases, 14(6), e0008335-e0008335 (2020-06-09)
Zika virus (ZIKV), a neglected tropical disease until its re-emergence in 2007, causes microcephaly in infants and Guillain-Barré syndrome in adults. Its re-emergence and spread to more than 80 countries led the World Health Organization in 2016 to declare a
R Mayor et al.
British journal of cancer, 100(10), 1534-1539 (2009-04-23)
Large chromosomal regions can be suppressed in cancer cells as denoted by hypermethylation of neighbouring CpG islands and downregulation of most genes within the region. We have analysed the extent and prevalence of long-range epigenetic silencing at 2q14.2 (the first
Liang-Yu Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(7), 1829-1834 (2016-02-03)
Spermatogonial stem cells (SSCs) are a subpopulation of undifferentiated spermatogonia located in a niche at the base of the seminiferous epithelium delimited by Sertoli cells and peritubular myoid (PM) cells. SSCs self-renew or differentiate into spermatogonia that proliferate to give

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