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Key Documents

R4132

Sigma-Aldrich

Reticulocyte Stain

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About This Item

UNSPSC Code:
41116121
NACRES:
NA.47

form

solution

Quality Level

shelf life

Expiry date on the label.

IVD

for in vitro diagnostic use

application(s)

hematology
histology

storage temp.

room temp

Application

Utilizes New Methylene Blue for the identification of reticulocytes in blood films. Reticulocyte Stain has been used in the detection of reticulocytes.

Principle

Blood and reticulocyte staining solution (New Methylene Blue) are mixed and incubated briefly at room temperature. Wedge or spun smears are made on microscope slides, air dried and evaluated under oil immersion on a light microscope. A red blood cell scoring positive would be observed containing two or more blue-stained granules.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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HDL cholesterol levels are an important factor for determining the lifespan of erythrocytes
Meurs I, et al.
Experimental Hematology, 33(11), 1309-1319 (2005)
Thiet M Vu et al.
Nature, 550(7677), 524-528 (2017-10-19)
Sphingosine-1-phosphate (S1P), a potent signalling lipid secreted by red blood cells and platelets, plays numerous biologically significant roles. However, the identity of its long-sought exporter is enigmatic. Here we show that the major facilitator superfamily transporter 2b (Mfsd2b), an orphan

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